2024 Click-it® edu alexa fluor® 488 imaging kit

Click-it® edu alexa fluor® 488 imaging kit

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August undefined, 2024

WebDesigned to label and detect incorporated EdU and perform cell cycle analysis on samples from adherent cells. Manufacturer: Invitrogen™ C10637. Catalog No. C10637. $732.00 / Each of 1. Qty Check Availability. Add to cart. Includes: EdU (5-ethynyl-2' -deoxyuridine), Alexa Fluor dye-picolyl azide, anhydrous dimethylsulfoxide (DMSO), Click-iT ... WebIn the next step, EdU-labeled newly synthesized DNA is imaged through a click reaction with best-in-class green fluorescent Azide 488 (Alexa Fluor 488® equivalent). Click-&-Go ® EdU 488 Cell Proliferation Assay …

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WebMar 9, 2015 · Click-iT® EdU Imaging Kits . Table 1. Contents and storage information. Material C10337. C10338 C10339: C10340 Concentration: ... • Protect from : light • DO … WebArticle Snippet: Then, click reaction was performed on indexed and mixed DNA for 30 minutes at RT under the following conditions: 1X Click-IT buffer (Click-iT EdU Alexa … head of customer service john lewis email

Click-iT® RNA Alexa Fluor® 488 Imaging Kit from Invitrogen

WebThe Click-iT EdU Alexa Fluor 488 Imaging Kit is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications. In this … WebInvitrogen™ Click-iT™ Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594 dye. Designed to label and detect incorporated EdU and perform cell cycle analysis on … WebNov 17, 2024 · Proceed essentially as instructed in the Click-iT ® EdU Alexa Fluor ® 488 Imaging Kit. 1. Freshly prepare the Click-iT ® reaction cocktail as instructed in the kit and apply it to each section for 30 min. 2. Wash slides with 3% BSA in PBS for 2 min, and then with PBS for 2 min (see Note 5). 3. head of customer service interview questions

Click-iT® EdU Flow Cytometry Assay Kits - Labettor

Alexa Fluor 488 Goat anti-mouse IgG minimal x-reactivity

WebProduct Details. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. WebThis trial-size kit provides sufficient EdU to label up to 6–30 coverslips and enough Alexa Fluor™ 488, 594, and 647 azide to detect new DNA synthesis on 2–10 coverslips with each fluorophore. • Simple—works the … head of customer service m\\u0026sWebThe Click-iT® EdU Alexa Fluor® 488 HCS Assay is a superior alternative to traditional proliferation assays that is optimized for high-content imaging applications. In this assay … goldrich security cabinets

"Webrabbit serum (A6455) and visualized with an Alexa Fluor® 488 goat anti-rabbit IgG antibody (A11034). Green fluorescence is also seen in the endoplasmic reticulum as it is formed … " - Click-it® edu alexa fluor® 488 imaging kit

Click-it® edu alexa fluor® 488 imaging kit

Click-iT™ EdU Imaging Kit with Alexa Fluor™ 488, 594, …

WebAlexa Fluor® 488 azide (Cat. no. C10425), Alexa Fluor® 647 azide (Cat. no. C10424), or Pacific Blue™ azide (Cat. no. C10418) (Component B) 1 vial NA Dimethylsulfoxide (DMSO) (Component C) 4.25 mL NA Click-iT® fixative (Component D) 5 mL 4% paraformaldehyde in PBS Click-iT® saponin-based permeabilization and wash reagent (Component E) WebMar 25, 2024 · Alexa Fluor 488-conjugated phalloidin (green) and DAPI (blue) were used to visualize actin cytoskeleton and nuclei, respectively. Red dots were counted in three random fields and quantitated. ... (Edu) staining was performed using the Click-iT™ EdU imaging kit (Invitrogen) according to the manufacturer's protocol. Edu (10 µM) was added into ...

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WebClick-iT™ EdU Imaging Kits Table 1. Contents and storage information. ... (Alexa Fluor® 488) 1 vial (Alexa Fluor® 594) 1 vial (Alexa Fluor® 647) NA Dimethylsulfoxide (DMSO, Component C) WebDesigned to label and detect incorporated EdU and perform cell cycle analysis on samples from adherent cells. Manufacturer: Invitrogen™ C10637. Catalog No. C10637. $732.00 / …

WebThe Click-iT EdU Cell Proliferation Kit for Imaging is a superior alternative to traditional proliferation assays that is optimized for fluorescence microscopy applications. In this … The Click-iT EdU Cell Proliferation Kit for Imaging is a superior alternative to … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebFeb 2, 2024 · 1,500 Beltone hearing centers. Unlike many other hearing aid brands, Beltone sells its products through its own retail stores. With 1,500 Beltone-branded …

WebThe alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to Alexa Fluor ® 488. The advantage of EdU labeling is that the small size of the dye azide allows for efficient ... WebApr 14, 2024 · Recently Concluded Data & Programmatic Insider Summit March 22 - 25, 2024, Scottsdale Digital OOH Insider Summit February 19 - 22, 2024, La Jolla

WebArticle Snippet: Then, click reaction was performed on indexed and mixed DNA for 30 minutes at RT under the following conditions: 1X Click-IT buffer (Click-iT EdU Alexa Fluor 488 Imaging Kit, Thermo Fisher, C10337), 0.5 mM picolyl-azide-PEG4-biotin (Jena Bioscience, CLK-1167-100), 0.1 mM CuSO4 (from Click-iT kit), 0.5 mM THPTA (Sigma, …

WebFeb 3, 2010 · The ability to detect newly synthesized RNA or global changes in RNA levels in a temporal manner is an important aspect of molecular biology; important changes in RNA can occur due to disease, environmental damage, or drug treatment. The Click-iT® RNA Alexa Fluor® 488 Imaging Kit from Invitrogen contains all the components needed to … head of customer service morrisonsWebFeb 3, 2010 · The ability to detect newly synthesized RNA or global changes in RNA levels in a temporal manner is an important aspect of molecular biology; important changes in … head of customer service m\u0026sWebThe BD Pharmingen™ 488 EdU Click Proliferation Kit contains 6-FAM Azide, which is excited by the blue laser and has an excitation maximum of 496 nm and an emission maximum of 516 nm. ... • FACS tubes or … head of customer service noc codeWebcontains the alkyne, while the Alexa Fluor® 488 dye, Alexa Fluor® 647 dye, or Pacific Blue™ dye contains the azide. Standard flow cytometry methods are used for determining the percentage of cells in the population that are in S-phase (Figures 1 and 4). The advantages of Click-iT™ EdU labeling are readily evident while performing the assay goldrich tubaWebGenistein diet does not modify crypt morphology in the ob/ob mouse jejunum: a comparison of cryostat and clearing techniques Noemy Sandoval-Skeet,1 Jason A Kaufman,2 Monica J Castro,2 Layla Al-Nakkash3 1Department of Biomedical Sciences, 2Department of Anatomy, 3Department of Physiology, Midwestern University, Glendale, AZ 85308, USA … head of customer service managementWebClick-iT EdU Alexa Fluor 594 Imaging Kit, Click-iT EdU Alexa Fluor 488 Imaging Kit green (ThermoFisher Scientific) (6) Vectashield mounting medium with DAPI (Vector Laboratories, Inc.) (7) Standard immunofluorescence (IF) analysis-related reagents (optional, for detection of EdU and protein of interests) (8) head of customer service londonWeb图 1.Pacific Blue™、Alexa Fluor 488 和 Alexa Fluor 647 Click-iT EdU 流式细胞检测试剂盒的荧光信号。使用 10 μM EdU 处理 Jurkat 细胞（人外周白血病 T 细胞） 2 小时，并按照推荐的染色方案进行检测。插图明显区分了掺入 EdU 的增殖细胞和未掺入 EdU 的非增殖细 … gold rich women