2024 D3922 thermo fisher scientific

D3922 thermo fisher scientific

Author: trqv

August undefined, 2024

Web493/503 (D3922; Thermo Fisher Scientific) for 30min, followed by thrice PBS washes. The IEC were observed with a laser scan- ... (Thermo Fisher Scientific) and blocked with 8% (w/v) skimmed milk in Tris buffered saline þ Tween 20 (TBST) buffer (20m M Tris–HCl, pH7·5,150mM sodiumchloride,0·1%Tween20)atroomtemper- WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 …

Zinc reduces hepatic lipid deposition and activates lipophagy via …

WebJan 1, 2024 · Shikonin was purchased from Selleck (S8279). Lipofectamine 3000 for siRNA transfection (Invitrogen), HilyMax for plasmid transfection (Dojindo, Kumamoto, Japan). Puromycin（Sigma，St. Louis, MO, USA). BODIPY 493/503 … WebJul 4, 2024 · They were cultured in Dulbecco’s Modified Eagle’s medium (Thermo Fisher Scientific) media containing 10% fetal bovine serum (FBS) (Thermo Fisher Scientific) in an incubator at 28℃. ... (D3922; Thermo Fisher Scientific) for 30 min, followed by thrice PBS washes. The IECs were observed using a laser scanning confocal microscope … held below the surface new world quest

Cells Free Full-Text Dietary Choline Mitigates High-Fat Diet ...

WebScientist at Pharmaceutical Product Development, a part of Thermo Fisher Scientific Ashburn, Virginia, United States. 120 followers 119 … WebNov 18, 2024 · DFCP1 is an ER-associated protein with two PI (3)P-binding FYVE domains. In WT cells, myc-DFCP1 formed very small punctate structures ( Figure 1E) ( Axe et al., 2008; Li et al., 2024 ). In SKO cells, the myc-DFCP1 structures appeared highly irregular and significantly enlarged ( Figure 1, E and F). WebWith its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar … With its nonpolar structure and long-wavelength absorption and … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes held below the surface new world bug

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Model: 26C3922-LS-TI - Faucet.com

WebJul 26, 2024 · BODIPY lipid staining of 3D-cultured HOF spheroids was performed as described previously. 29, 31, 32 Briefly, 4% paraformaldehyde-fixed 3D spheroids were incubated in a mixture of 0.1% BODIPY (#D3922; Thermo Fisher Scientific), 0.1% DAPI (#D523; Doujin), and 0.1% phalloidin (#20553; Funakoshi) in PBS containing 3% bovine … WebOct 21, 2024 · LipidSearch™ (Thermo Fisher Scientific) was used to make lipid peak assignments in the single cells. Only peaks above a 1E4 intensity threshold and within 5 ppm of the calculated m / z value were selected. ... (#D3922, Invitrogen, USA) in PBS and incubated in the dark for 15 minutes at 37 °C with 5% CO 2. After incubation, cells were … held below the surface quest new worldWebIn order to identify podocyte proteins that could perform as a similar or better biomarker for the presence of CPF (s), or are involved in the mechanism of action of CPF (s), we here followed a proteomics approach by analyzing proteomes of hPod exposed to presumably CPF-containing plasmas from patients with FSGS. held below the surface quest

"WebFeb 1, 2024 · We performed ORO and Bodipy 493/503 (D3922; Thermo Fisher Scientific, Waltham, MA, USA) staining based on the protocols described in our previous research [4, 13]. The ten fields were selected from each sample to qualify relative areas of LDs in ORO by Image J. We used the laser scanning confocal microscope (Leica Microsystems, … " - D3922 thermo fisher scientific

D3922 thermo fisher scientific

Nanocapillary sampling coupled to liquid chromatography mass ...

WebFeb 9, 2024 · To visualize the accumulation of lipids in response to OA treatment, we used boron-dipyrromethene (BODIPY) 493/503 (D3922, Thermo Fisher Scientific, MA, USA), which labels specifically ... WebJan 22, 2024 · The level of LDs was assayed using BODIPY 493/503 (#D3922, Thermo Fisher Scientific) according to the manufacturer's protocol. 2.5. Western blot. Western blot was performed as previously described [24]. In brief, proteins in the cell lysate or supernatants were resolved on 4%–12% Criterion XT Bis-Tris gels (#3450124, Bio-Rad, …

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WebMeasurement Range: -50 to +392°F/-45 to +200°C NSF® Certified One-button operation 3 mm tip 5"/12.7 cm stem Shatterproof Food-safe ABS plastic with BioCote® Stainless … WebJan 9, 2024 · The BODIPY 493/503 (D3922; Thermo Fisher Scientific, USA) staining was performed for primary IECs, as described by Wei et al. . The laser scanning confocal microscope (Leica Microsystems, Germany) …

Web货号: D3922 相关应用: Cellular Imaging 技术支持客户服务收藏夹产品概述推荐产品文件常见问题解答引用和文献 BODIPY™ 493/503 具有非极性结构、吸收波长较长且发荧 … WebBioactive Small Molecules D3922 Print Invitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza- s -Indacene) …

WebMay 14, 2024 · To analyze their lipid droplet formation for the 3D culture, the organoids were transferred to 6 super-low attachment well dishes, incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific) in ... WebApr 20, 2024 · To assess the viability of the adipocytes on-chip, a live/dead-assay based on fluorescein diacetate (FDA; F1303, Thermo Fisher Scientific) and propidium iodide (PI; P3566, Thermo Fisher Scientific ...

WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 …

WebJul 23, 2024 · Thermo Fisher: Cat #D3922: Donkey anti-mouse IgG (H + L) secondary antibody alexa Fluor® 488 conjugate: Thermo Fisher: ... (D3922, Thermo Fisher Scientific, California, USA) staining solution in the dark for 15 min at 37°C. After washing 3 times with PBS, cells were stained with theirs surface markers for 30min at 4°C. Data … heldb horaireWebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 … held black bobWebMay 14, 2024 · Most recently, to understand the molecular etiology of thyroid-associated orbitopathy, our group utilized the 3D cell culture of human orbital fibroblasts, and found that hypoxia-inducible factor... held biker fashionWebDec 12, 2024 · Lipophilic green-fluorescent BODIPY 493/503 (D3922, Thermo Fisher Scientific, Waltham, MA, USA) was used for lipid staining. SkMs were seeded at a density of 1000 cells/cm 2 and treated as indicated. held biker fashion sonthofenWebSU24 - Scientific research and development Uses advised againstNot for consumer use. Details of the supplier of the safety data sheet Manufacturer/Supplier 24 hour Emergency … held boltshausenWebJan 16, 2024 · Briefly, 2D or 3D 3T3-L1 cells were incubated in a mixture of 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA), Alexa Fluor 594 phalloidin (# 20553, Funakoshi, Tokyo, Japan), and DAPI (# D523, Dojindo, Kumamoto, Japan), at 1:1000 dilutions in PBS for 1 hr, and thereafter fixed in 4% paraformaldehyde in PBS for … held boys philatelicsWebFeb 24, 2024 · Finally, BODIPY493/503 (#D3922, Thermo Fisher Scientific, USA) staining was performed to check the lipid droplet formation. ... USA) according to the manufacturer’s instruction. SYBR Green qPCR Master Mix (#K0251, Thermo Fisher Scientific, USA) was used for quantitative real-time PCR amplification with a CFX96 real-time PCR detection … held biker fashion australia