WebMaize is an important food and feed crop in many countries. It is also one of the most important target crops for the application of biotechnology. Currently, there are more … WebDec 1, 2024 · Genotype dependence in plant transformation means that one can only achieve genetic transformation on limited numbers of plant species or varieties. In many instances, a successful protocol for one plant variety may not be transferrable to another one. For example, the protocol for maize inbred B104 cannot be applied to B73 [5]. …
(PDF) Handbook of Maize - ResearchGate
WebThis chapter describes two transformation protocols using Agrobacterium- and biolistic-mediated methods for gene delivery. Immature zygotic embryos of maize inbred B104, excised from ears harvested 10-14 days post pollination, … WebAug 16, 2002 · Methods for Agrobacterium-mediated transformation of maize followed the general protocol described with the following modifications. Agrobacteria were grown to log phase in liquid minimal A medium containing 100 μM spectinomycin. ... The increase in maize transformation could be caused by enhanced transgene integration. If this were … free murphy bed plans download
Biolistic DNA Delivery in Maize Immature Embryos
WebIn this chapter, we describe the Agrobacterium-mediated maize transformation protocol used routinely in our laboratory ( 6) to transform immature embryos of the maize Hi II genotype. A. tumefaciens strain EHA101 harboring a standard binary vector is used to infect the embryos and deliver transgenes into plant cells. WebApr 1, 2024 · Maize is a versatile cereal crop having the highest genetic potential, production, and productivity. In the past few decades, plant tissue culture and transformation approaches have played an... WebFeb 14, 2024 · Demonstrated here is a detailed protocol for Agrobacterium-mediated genetic transformation of maize inbred lines using morphogenic genes Baby boom (Bbm) and Wuschel2 (Wus2). Bbm is regulated by the maize phospholipid transferase gene (Pltp) promoter, and Wus2 is under the control of a maize auxin-inducible (Axig1) promoter. free murphy door plans