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Phic31 integrase expression plasmid

WebJan 1, 2007 · The phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid … WebGet all the plasmids you need for PinPoint Targeted Integration using the PhiC31 Integrase to place the PinPoint attP site into the target genome The PinPoint-FC attP Placement Vector (# PIN300A-1) The PhiC31 Integrase (# FC200PA-1) The PinPoint Integrase Expression Plasmid (# PIN200A-1) The CAG PinPoint Donor Vector (# PIN510A-1)

Method of controlling cellular processes in plants

WebSBI’s PhiC31 Integrase System consists of the PhiC31 Integrase Expression Plasmid and a PhiC31 Donor Plasmid that has your gene-of-interest cloned in. The system enables: Non … Lentiviral Expression Plasmids & Lentiviral Vectors - PhiC31 Integrase Expression … Use the form below and we’ll get back to you within 24-hours, or call us toll free at … Exosome Isolation Kits & Products - PhiC31 Integrase Expression Plasmid System … How it works. Ordering NTA-based Exosome Characterization Services is … Exosome Quantitation Kits & Products - PhiC31 Integrase Expression Plasmid … Lentiviral Expression Plasmids & Lentiviral Vectors Whatever type of cell you’re … CLOuD9 Gene Expression Regulation System; Targeted Protein Degradation; … How it works. Ordering RNA Services is easy. Step 1: Contact the services team … Exosome Biomarker Discovery Products - PhiC31 Integrase Expression Plasmid … Get everything you need for studying and using exosomes, from isolation and … WebOct 28, 2024 · In one exemplary embodiment, the method disclosed herein selects a variant integrase (e.g., a phiC31 integrase) that can recognize a mutant attP sequence (e.g., SEQ ID NO: 3). The target mutant attP sequence is different from the wildtype attP site in more than 50% of the nucleotide residues, e.g., 26 out of 48 nucleotide residues are different. penn plax small world filter https://bearbaygc.com

Efficient reversal of phiC31 integrase recombination in …

WebpcDNA3.1 phiC31 (Plasmid #68310) Print Enlarge View all sequences Purpose In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection). Depositing Lab … WebMay 7, 2013 · The resulting plasmid possessed the phiC31-integrase target site attP and a marker gene, 3xP3DsRed, between the arms of piggyBac that led to the expression of red fluorescent protein in the stemmata of embryos and the eyes of adults (Fig. 1a). WebThe phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid DNA into … penn-plax curved corner glass aquarium kit

Pinpoint & PhiC31 Integrase Systems System Biosciences

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Phic31 integrase expression plasmid

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WebpTFPhiC31 Plasmid #139671 Purpose Expression cassette for phiC31 Integrase in T-DNA vector Depositor James Birchler Insert phiC31 Integrase Use Tags Expression Plant Mutation Promoter Maize Ubiquitin Availability Academic Institutions and Nonprofits only Enlarge pBT317_pET-PhiC31o-pA Plasmid #52491 Purpose for preparation of PhiC31o … WebMay 13, 2024 · Splicing is an important RNA processing step. Genetic variations can alter the splicing process and thereby contribute to the development of various diseases. Alterations of the splicing pattern can be examined by gene expression analyses, by computational tools for predicting the effects of genetic variants on splicing, and by …

Phic31 integrase expression plasmid

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WebFeb 28, 2014 · Thyagarajan et al. 5 demonstrated that phiC31 integrase can mediate attB-containing plasmid integration in an unmodified mammalian genome with an increased … WebOct 17, 2005 · The phiBT1 phage integrase is an enzyme that catalyses the efficient recombination between unique sequences in the phage and bacterial genomes, leading to the site-specific integration of the former into the latter in a unidirectional manner.

WebAug 1, 2014 · Int-φC31 activity assay plasmids. Plasmid pCMV-Int for expression of the integrase in mammalian cells was supplied by Dr. M.P. Calos of Stanford University (Groth et al., 2000).The attB sequence was from pBCPB (provided by Dr. M. P. Calos) and cloned into the AflII site of pEGFP-N1 to create the plasmid pEGFP-N1-attB, which contained the … WebJul 22, 2015 · PhiC31 integrase is a Streptomyces phage recombinase, which can mediate recombination between the bacterial attachment site attB and the phage attachment site attP (Kudriavtseva et al. 1994; Thorpe and Smith 1998 ).

WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide …

WebJun 12, 2024 · In most prior studies, phiC31 integrase has been introduced as plasmid DNA or mRNA. The current report examines whether phiC31 integrase functions efficiently in …

WebpUPD2 PhiC31 integrase (GB1561) was a gift from Diego Orzaez (Addgene plasmid # 160586 ; http://n2t.net/addgene:160586 ; RRID:Addgene_160586) For your References section: A memory switch for plant synthetic biology based on the phage varphiC31 integration system. penn plax cat walk litter box enclosureWebThe PhiC31 Integrase System Frequently used for gene therapy development and related applications Non-viral transgene delivery Single-step process Single-copy integration … penn plax smallworld filter instructionsWeb1. A plasmid comprising the following units: (a) an endonuclease restriction site,(b) a promotorless endonuclease expression cassette,(c) a promoter that is not operably linked to the endonuclease expression cassette,(d) a sequence coding for an enzyme that catalyzes site-specific recombination,(e) at least two recognition sequences for the enzyme … penn plax spin kitty cat wheeltoaster oven meals for lunchesWebPlasmid pUPD2 PhiC31 integrase (GB1561) from Dr. Diego Orzaez's lab contains the insert PhiC31 and is published in Nucleic Acids Res. 2024 Apr 6;48(6):3379-3394. doi: … penn-plax spin kitty cat wheelWebJun 12, 2024 · In most prior studies, phiC31 integrase has been introduced as plasmid DNA or mRNA. The current report examines whether phiC31 integrase functions efficiently in mammalian cells when co-nucleofected as a purified protein, along with attB-containing donor plasmids or PCR fragments. penn-plax medium bird kit square bird cageWebWith the PhiC31 Integrase System, you simply clone your gene-of-interest into the attB Donor Plasmid, and then (Step 1) co-transfect with a PhiC31 Integrase Expression Plasmid. (Inside the cell) The PhiC31 Integrase is transiently expressed, and mediates site-specific recombination between the attB site on the donor plasmid and a pseudo attP ... penn plax hob power filter