Phic31 integrase system
WebThe system is based on that integrase (PhiC31) catalyzes irreversible recombination between attB and attP sites. We developed integrase-mediated site-specific transgenesis in mice via pronuclear microinjection. WebMay 7, 2013 · We showed in our previous paper that phiC31 integrase works very efficiently in silkworm embryos using an extra chromosomal cassette exchange reaction system; recombination between the attP and attB sites of the plasmid occurred with a high frequency in the silkworm embryos when phiC31-integrase mRNA was supplied by injection and no …
Phic31 integrase system
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WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … WebJul 29, 2012 · Notably, a phiC31 integrase mRNA synthesized from two of the plasmids produced cassette exchange plasmids at a high frequency, suggesting that the mRNA can be used to construct a targeted integration system in silkworms. Introduction Transgenic organisms are very useful tools to analyze gene function.
WebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, … WebMar 20, 2024 · The phiC31 integrase is a member of serine-catalyzed enzymes encoded by this phage and mediates the aforementioned phage genome insertion(Smith and Thorpe Citation2002). This enzyme inserts exogenous DNA into a particular location by using the recognition of two sequences.
WebAug 1, 2014 · The phiC31 integrase system for gene therapy. Curr Gene Ther 6,633–645 [Google Scholar] Chalberg T.W., Genise H.L., Vollrath D., and Calos M.P. (2005). phiC31 integrase confers genomic integration and long-term transgene expression in rat retina. Invest Ophthalmol Vis Sci 46,2140–2146 [Google Scholar] WebJun 17, 2010 · In addition to the widely used Cre and Flp recombinases, the phiC31 integrase system from Streptomyces phage may also be used for these genetic manipulations in eukaryotic cells. Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination.
WebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB …
WebThis new feature of the integrase system gives it more utility and flexibility for making changes in chromosomes. In addition, in this study we created a new plasmid, or circle of DNA, that codes for a useful form of phiC31 integrase. The new plasmid, called pCS-kI, encodes a slightly shorter form of integrase. the vikings mc seriesWebMay 7, 2013 · It is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific … the vikings las vegasWebThe phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid DNA into … the vikings ivar the bonelessWebSep 14, 2012 · The Streptomyces phage phiC31 integrase was tested for its feasibility in excising transgenes from the barley genome through site-specific recombination. We produced transgenic barley plants expressing an active phiC31 integrase and crossed them with transgenic barley plants carrying a target locus for recombination. The target … the vikings movie 1958 castWebDec 1, 2006 · The phiC31 integrase can integrate introduced plasmid DNA into preferred locations in unmodified mammalian genomes, resulting in robust, long-term expression of … the vikings movie trailerWebPlasmid pcDNA3.1 phiC31 from Dr. Konrad Basler's lab contains the insert phiC31 and is published in Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3312-7. ... integrase Alt name. serine family SSR phiC31 integrase Insert Size (bp) ... An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. the vikings movie youtubeWebApr 10, 2024 · The phiC31 integrase functions efficiently in vitro and in Escherichia coli, yeast, and mammalian cells, mediating unidirectional site-specific recombination between its attB and attP recognition ... the vikings movie cast