Targeted mutagenesis in zebrafish by talens
WebOct 27, 2016 · In zebrafish, two other targeted mutagenesis tools, ZFNs and TALENs, are being used 18. We compared the germline transmission efficiencies of ZFNs, TALENs and CRISPR/Cas9 in zebrafish.
Targeted mutagenesis in zebrafish by talens
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WebSep 4, 2014 · Sood R, Carrington B, Bishop K, Jones M, Rissone A, et al. (2013) Efficient methods for targeted mutagenesis in zebrafish using zinc-finger nucleases: data from targeting of nine genes using CompoZr or CoDA ZFNs. PloS one 8: e57239. [PMC free article] [Google Scholar] WebNov 21, 2013 · We previously described ZFN-mediated targeted mutagenesis and insertion of reporter genes in sea urchin embryos. In this study, we demonstrate that TALENs can induce mutagenesis at specific genomic loci of sea urchin embryos. ... 2012, 2013), zebrafish (Doyon et al. 2008; Meng et al. 2008; Hisano et al. 2013), frogs (Young et al.
WebFeb 22, 2013 · Recently, it has been shown that targeted mutagenesis using zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can be used to generate knockout zebrafish lines for analysis of their function and/or developing disease models. A number of different methods have been developed for the design and assembly … WebJan 1, 2016 · Targeting the zebrafish genome with zinc-finger nucleases was the beginning of a new era in zebrafish biology, as selected genes could finally be specifically targeted …
WebSep 23, 2012 · Here we show that improvements in artificial transcription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zebrafish genome editing and functional genomic ... WebFeb 24, 2013 · We therefore reasoned that TALENs may be useful for targeted knock-in through homologous recombination in zebrafish as well. We constructed a TALEN pair targeting exon 4 of the zebrafish th gene.
WebMay 24, 2012 · TALENs exhibited similar toxicity to CoDA ZFNs, with >50% of injected animals surviving to 3 days of life. Taken together, our results suggest that TALEN …
WebJan 7, 2013 · The higher mutation rates and ability to target essentially any sequence make TALENs the superior technology for targeted mutagenesis in zebrafish, and likely other animal models. INTRODUCTION Zinc-finger nucleases (ZFNs) and TAL effector nucleases (TALENs) have recently emerged as powerful tools for generating targeted genomic … job of epithelial tissueWebOct 19, 2024 · The zebrafish was originally chosen as a model with forward genetics in mind and has been used successfully in countless genetic screens. However, it quickly became … insulated coats mens streetWebFeb 21, 2024 · In addition to CRISPR/Cas9, Transcription Activator-like Effector Nuclease(TALENS), and Zinc Finger Nucleases (ZFNs) are other targeted mutagenesis methods being used to generate knockouts for the ... job of executive chefWebAug 1, 2011 · TALENs may offer potential advantages over ZFNs for mutagenesis of genes in zebrafish and other model organisms such as Caenorhabditis elegans 12, because they … job of epidermisWebTargeted Mutagenesis in Zebrafish by TALENs Authors: Peng Huang 1 , An Xiao 1 , Xiangjun Tong 1 , ... Ivey J, Zonies S, Wu X, Wu K, Yang H, Meng Q, Zhu Z, Zhang B, Lin S, Burgess … job of executive branchWebAug 5, 2011 · In the 2000s new "one-size-fits-all" gene targeting technologies, including TALENs and Zinc finger nucleases, allowed targeted mutagenesis in a greater variety of genetic models, such as zebrafish ... insulated coats girlsWebApr 17, 2014 · To overcome these drawbacks, it is now welladmitted in the zebrafish community that a targeted-mutagenesis assay must be preferred, such as Zinc Finger Nucleases (Doyon et al., 2008), TALEN (Hwang ... job of environmental safeguard october 2022